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Insight into GFPmut2 pH Dependence by Single Crystal Microspectrophotometry and X-ray Crystallography.

The journal of physical chemistry. B - 13 Dec 2018

Lolli Graziano, Raboni Samanta, Pasqualetto Elisa, Benoni Roberto, Campanini Barbara, Ronda Luca, Mozzarelli Andrea, Bettati Stefano, Battistutta Roberto

Abstract excerpt

The fluorescence of Green Fluorescent Protein (wtGFP) and variants has been exploited in distinct applications in cellular and analytical biology. GFPs emission depends on the population of the protonated (A-state) and deprotonated (B-state) forms of the chromophore. Whereas wtGFP is pH-independent, mutants in which Ser65 is replaced by either threonine or alanine (as in GFPmut2) are pH-dependent, with a p Ka...

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Crystallography, X-Ray
Escherichia coli
Green Fluorescent Proteins
Hydrogen Bonding
Hydrogen-Ion Concentration

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Binding

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PMID: 30179482
DOI: 10.1021/acs.jpcb.8b07260

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