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Minimum set of mutations needed to optimize cyan fluorescent proteins for live cell imaging.

Molecular bioSystems - 2 Feb 2013

Erard Marie, Fredj Asma, Pasquier Hélène, Beltolngar Dahdjim-Benoît, Bousmah Yasmina, Derrien Valérie, Vincent Pierre, Merola Fabienne

Abstract excerpt

Cyan fluorescent proteins (CFPs) are widely used as FRET donors in genetically encoded biosensors for live cell imaging. Recently, cyan variants with greatly improved fluorescence quantum yields have been developed by large scale random mutagenesis. We show that the introduction of only two mutations, T65S and H148G, is able to confer equivalent performances on the popular form ECFP, leading to Aquamarine (QY =...

Topics

Amino Acid Substitution
Animals
Cell Line
Cricetinae
Dogs
Escherichia coli
Fluorescence Resonance Energy Transfer
Green Fluorescent Proteins
Madin Darby Canine Kidney Cells
Microscopy, Fluorescence

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Imaging

Mutation

Recombinant Proteins

Source

Identifiers

PMID: 23192565
DOI: 10.1039/c2mb25303h

Genes

CFP

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