Article
Differential manipulation of arrestin-3 binding to basal and agonist-activated G protein-coupled receptors.
Cellular signalling - 1 Aug 2017
Prokop Susanne, Perry Nicole A, Vishnivetskiy Sergey A, Toth Andras D, Inoue Asuka, Milligan Graeme, Iverson Tina M, Hunyady Laszlo, Gurevich Vsevolod V
Abstract excerpt
Non-visual arrestins interact with hundreds of different G protein-coupled receptors (GPCRs). Here we show that by introducing mutations into elements that directly bind receptors, the specificity of arrestin-3 can be altered. Several mutations in the two parts of the central "crest" of the arrestin molecule, middle-loop and C-loop, enhanced or reduced arrestin-3 interactions with several GPCRs in receptor...
Topics
- Amino Acid Sequence
- Animals
- Arrestins
- COS Cells
- Cattle
- Chlorocebus aethiops
- Conserved Sequence
- HEK293 Cells
- Humans
- Lysine
- Mutant Proteins