The role of individual residues in arrestin-3 finger loop in its binding to β2-adrenergic receptor.

Abstract excerpt

Arrestins preferentially bind active phosphorylated forms of their cognate G-protein-coupled receptors (GPCRs). The finger loop inserts into the interhelical cytoplasmic cavity of activated receptors and has been proposed to serve as an activation sensor. We mutagenized the finger loop (residues 63-78) in bovine arrestin-3 and measured the binding to the β2-adrenergic receptor (β2AR) in living cells. Only one...

Topics

• Animals
• Receptors, Adrenergic, beta-2
• Cattle
• Protein Binding
• Humans
• Arrestins
• Amino Acid Sequence