Article
Functional map of arrestin binding to phosphorylated opsin, with and without agonist.
Scientific reports - 28 Jun 2016
Peterhans Christian, Lally Ciara C M, Ostermaier Martin K, Sommer Martha E, Standfuss Jörg
Abstract excerpt
Arrestins desensitize G protein-coupled receptors (GPCRs) and act as mediators of signalling. Here we investigated the interactions of arrestin-1 with two functionally distinct forms of the dim-light photoreceptor rhodopsin. Using unbiased scanning mutagenesis we probed the individual contribution of each arrestin residue to the interaction with the phosphorylated apo-receptor (Ops-P) and the agonist-bound form...
Topics
- Animals
- Arrestin
- Cattle
- Models, Molecular
- Mutation
- Opsins
- Protein Binding
- Protein Domains