Article
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.
Nature genetics - 1 Apr 2017
Canver Matthew C, Lessard Samuel, Pinello Luca, Wu Yuxuan, Ilboudo Yann, Stern Emily N, Needleman Austen J, Galactéros Frédéric, Brugnara Carlo, Kutlar Abdullah, McKenzie Colin, Reid Marvin, Chen Diane D, Das Partha Pratim, A Cole Mitchel, Zeng Jing, Kurita Ryo, Nakamura Yukio, Yuan Guo-Cheng, Lettre Guillaume, Bauer Daniel E, Orkin Stuart H
Abstract excerpt
Cas9-mediated, high-throughput, saturating in situ mutagenesis permits fine-mapping of function across genomic segments. Disease- and trait-associated variants identified in genome-wide association studies largely cluster at regulatory loci. Here we demonstrate the use of multiple designer nucleases and variant-aware library design to interrogate trait-associated regulatory DNA at high resolution. We developed a...
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