Article
Transcription factor networks disproportionately enrich for heritability of blood cell phenotypes.
Science (New York, N.Y.) - 4 Apr 2025
Martin-Rufino Jorge Diego, Caulier Alexis, Lee Seayoung, Castano Nicole, King Emily, Joubran Samantha, Jones Marcus, Goldman Seth R, Arora Uma P, Wahlster Lara, Lander Eric S, Sankaran Vijay G
Abstract excerpt
Most phenotype-associated genetic variants map to noncoding regulatory regions of the human genome, but their mechanisms remain elusive in most cases. We developed a highly efficient strategy, Perturb-multiome, to simultaneously profile chromatin accessibility and gene expression in single cells with CRISPR-mediated perturbation of master transcription factors (TFs). We examined the connection between TFs,...
Read the complete abstract on PubMedTopics
- Humans
- Transcription Factors
- Gene Regulatory Networks
- Chromatin
- Phenotype
- Single-Cell Analysis