Article

Novel enzyme-based reduced representation method for DNA methylation profiling with low inputs

2024-05-27

Abstract excerpt

DNA methylation at cytosine-phospho-guanine (CpG) residues is a vital biological process that regulates cell identity and function. Although widely used, bisulfite-based cytosine conversion procedures for DNA methylation sequencing require high temperature and extreme pH, which lead to DNA degradation, especially among unmethylated cytosines. Enzymatic methylation sequencing (EM-seq), an enzyme-based cytosine conv...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 07d24cac-e351-5c76-9ed5-f077380de859
DOI: 10.1101/2024.05.24.595803

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Novel enzyme-based reduced representation method for DNA methylation profiling with low inputsDOI 10.1101/2024.05.24.595803Read

Targeted alignment and end repair elimination increase alignment and methylation measure accuracy for reduced representation bisulfite sequencing data.Semantic proximity Read

Differential methylation analysis of reduced representation bisulfite sequencing experiments using edgeRSemantic proximity Read

GeneDMRs: an R package for Gene-based Differentially Methylated Regions analysisSemantic proximity Read

Cost-effective solutions for high-throughput enzymatic DNA methylation sequencingSemantic proximity Read

Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction.Semantic proximity Read

Investigating the potential of single-cell DNA methylation data to detect allele-specific methylation and imprinting.Semantic proximity Read

Comparing methylation levels assayed in GC-rich regions with current and emerging methodsSemantic proximity Read

Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells.Semantic proximity Read

Ultradeep bisulfite sequencing analysis of DNA methylation patterns in multiple gene promoters by 454 sequencing.Semantic proximity Read

Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAsSemantic proximity Read

Discovery of novel DNA cytosine deaminase activities enables a nondestructive single-enzyme methylation sequencing method for base resolution high-coverage methylome mapping of cell-free and ultra-low input DNASemantic proximity Read

Evolution of DNA methylome from precancerous lesions to invasive lung adenocarcinomasSemantic proximity Read