Article
Analysis of gene induction and arrest site transcription in yeast with mutations in the transcription elongation machinery.
The Journal of biological chemistry - 13 Apr 2001
Wind-Rotolo M, Reines D
Abstract excerpt
In vitro, transcript elongation by RNA polymerase II is impeded by DNA sequences, DNA-bound proteins, and small ligands. Transcription elongation factor SII (TFIIS) assists RNA polymerase II to transcribe through these obstacles. There is however, little direct evidence that SII-responsive arrest sites function in living cells nor that SII facilitates readthrough in vivo. Saccharomyces cerevisiae strains lacking...
Topics
- Adenosine Triphosphatases
- Base Sequence
- Blotting, Northern
- Cation Transport Proteins
- DNA Primers
- Fungal Proteins
- Mediator Complex
- Mutation
- Plasmids
- RNA Polymerase II
- RNA, Messenger
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sodium-Potassium-Exchanging ATPase
- Trans-Activators
- Transcription, Genetic
- Uracil