Article
Application of sorting and next generation sequencing to study 5΄-UTR influence on translation efficiency in Escherichia coli.
Nucleic acids research - 7 Apr 2017
Evfratov Sergey A, Osterman Ilya A, Komarova Ekaterina S, Pogorelskaya Alexandra M, Rubtsova Maria P, Zatsepin Timofei S, Semashko Tatiana A, Kostryukova Elena S, Mironov Andrey A, Burnaev Evgeny, Krymova Ekaterina, Gelfand Mikhail S, Govorun Vadim M, Bogdanov Alexey A, Sergiev Petr V, Dontsova Olga A
Abstract excerpt
Yield of protein per translated mRNA may vary by four orders of magnitude. Many studies analyzed the influence of mRNA features on the translation yield. However, a detailed understanding of how mRNA sequence determines its propensity to be translated is still missing. Here, we constructed a set of reporter plasmid libraries encoding CER fluorescent protein preceded by randomized 5΄ untranslated regions (5΄-UTR)...
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