Article
Effects of additional sequences directly downstream from the AUG on the expression of GFP gene.
Biochimica et biophysica acta - 30 Nov 2003
Zhao Kong-Nan, Tomlinson Liz, Liu Wen Jun, Gu Wenyi, Frazer Ian H
Abstract excerpt
We have studied the expression of the green fluorescent protein (GFP) gene to gain more understanding of the effects of additional nucleotide triplets (codons) downstream from the initiation codon on the translation of the GFP mRNA in CHO and Cos1 cells. A leader sequence of six consecutive identical codons (GUG, CUC, AGU or UCA) was introduced into a humanized GFP (hm gfp) gene downstream from the AUG to produce...
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