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Article

Directed evolution study unveiling key sequence factors that affect translation efficiency in Escherichia coli.

Journal of bioscience and bioengineering - 1 Nov 2013

Tsukuda Miyuki, Miyazaki Kentaro

Abstract excerpt

Synonymous mutations in protein coding genes significantly impact translation efficiency. We synthesized a pair of genes encoding green fluorescent protein that were separated by 160 synonymous mutations to investigate key factors that affect translation efficiency. One sequence was optimized for Escherichia coli (GFP(Eco)) and the other for Bacillus subtilis (GFP(Bsu)). When the genes were expressed in E. coli,...

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PMID: 23790548
DOI: 10.1016/j.jbiosc.2013.05.013

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AGA

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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