Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery.

Nucleic acids research - 1 Dec 2002

Ilegems Erwin, Pick Horst M, Vogel Horst

Abstract excerpt

A reporter assay was developed to detect and quantify nonsense codon suppression by chemically aminoacylated tRNAs in mammalian cells. It is based on the cellular expression of the enhanced green fluorescent protein (EGFP) as a reporter for the site-specific amino acid incorporation in its sequence using an orthogonal suppressor tRNA derived from Escherichia coli. Suppression of an engineered amber codon at...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Acylation
Share to Amino Acyl-tRNA Synthetases
Share to Codon, Terminator

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fluorescence Recovery After Photobleaching

Share to Luminescent Proteins

Share to Microinjections

Share to RNA, Transfer

Share to Suppression, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 12466560
DOI: 10.1093/nar/gnf128
PMCID: PMC137983

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Monitoring mis-acylated tRNA suppression efficiency in mammalian cells via EGFP fluorescence recovery.PMID 12466560Read ↗

Amber suppression in mammalian cells dependent upon expression of an Escherichia coli aminoacyl-tRNA synthetase gene.Semantic proximity Read ↗

Therapeutic promise of engineered nonsense suppressor tRNAs.Semantic proximity Read ↗

Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.Semantic proximity Read ↗

Increased expression of tryptophan and tyrosine tRNAs elevates stop codon readthrough of reporter systems in human cell lines.Semantic proximity Read ↗

Complete set of orthogonal 21st aminoacyl-tRNA synthetase-amber, ochre and opal suppressor tRNA pairs: concomitant suppression of three different termination codons in an mRNA in mammalian cells.Semantic proximity Read ↗

Efficient suppression of endogenous CFTR nonsense mutations using anticodon-engineered transfer RNAsSemantic proximity Read ↗

Optimization of Anticodon Edited Transfer RNAs (ACE-tRNAs) Function in Translation for Suppression of Nonsense MutationsSemantic proximity Read ↗

Modulation of efficiency of translation termination in Saccharomyces cerevisiae.Semantic proximity Read ↗

Enhanced yield of recombinant proteins with site-specifically incorporated unnatural amino acids using a cell-free expression system.Semantic proximity Read ↗

Development of amber suppressor tRNAs appropriate for incorporation of nonnatural amino acids.Semantic proximity Read ↗

High-fidelity and differential nonsense suppression in live cells and a frontotemporal dementia allele with human transfer RNAs.Semantic proximity Read ↗

Suppression of nonsense mutations in cell culture and mice by multimerized suppressor tRNA genes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.