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Article

Coding-sequence determinants of gene expression in Escherichia coli.

Science (New York, N.Y.) - 10 Apr 2009

Kudla Grzegorz, Murray Andrew W, Tollervey David, Plotkin Joshua B

Abstract excerpt

Synonymous mutations do not alter the encoded protein, but they can influence gene expression. To investigate how, we engineered a synthetic library of 154 genes that varied randomly at synonymous sites, but all encoded the same green fluorescent protein (GFP). When expressed in Escherichia coli, GFP protein levels varied 250-fold across the library. GFP messenger RNA (mRNA) levels, mRNA degradation patterns, and...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 19359587
DOI: 10.1126/science.1170160
PMCID: PMC3902468

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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