Article
Phased diploid genome assembly with single-molecule real-time sequencing.
Nature methods - 1 Dec 2016
Chin Chen-Shan, Peluso Paul, Sedlazeck Fritz J, Nattestad Maria, Concepcion Gregory T, Clum Alicia, Dunn Christopher, O'Malley Ronan, Figueroa-Balderas Rosa, Morales-Cruz Abraham, Cramer Grant R, Delledonne Massimo, Luo Chongyuan, Ecker Joseph R, Cantu Dario, Rank David R, Schatz Michael C
Abstract excerpt
While genome assembly projects have been successful in many haploid and inbred species, the assembly of noninbred or rearranged heterozygous genomes remains a major challenge. To address this challenge, we introduce the open-source FALCON and FALCON-Unzip algorithms (https://github.com/PacificBiosciences/FALCON/) to assemble long-read sequencing data into highly accurate, contiguous, and correctly phased diploid...
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