Article
De novo diploid genome assembly using long noisy reads.
Nature communications - 5 Apr 2024
Nie Fan, Ni Peng, Huang Neng, Zhang Jun, Wang Zhenyu, Xiao Chuanle, Luo Feng, Wang Jianxin
Abstract excerpt
The high sequencing error rate has impeded the application of long noisy reads for diploid genome assembly. Most existing assemblers failed to generate high-quality phased assemblies using long noisy reads. Here, we present PECAT, a Phased Error Correction and Assembly Tool, for reconstructing diploid genomes from long noisy reads. We design a haplotype-aware error correction method that can retain heterozygote...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.