Article

Multiplex mapping of protein-protein interaction interfaces

2025-01-29

Abstract excerpt

We describe peptide mapping through Sp lit A ntibiotic R esistance C omplementation (SpARC-map), a method to identify the probable interface between two interacting proteins. Our method is based on in vivo affinity selection inside a bacterial host, and uses high throughput DNA sequencing to infer the probable protein-protein interaction (PPI) interfaces. SpARC-map uses only routine microbiology techniques, wi...

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Literature Corpus work: 6867fe17-ce4f-542a-a8ea-4e732d7209a4
DOI: 10.1101/2025.01.28.635392

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