Article
Identification of oncogenic driver mutations by genome-wide CRISPR-Cas9 dropout screening.
BMC genomics - 9 Sept 2016
Kiessling Michael K, Schuierer Sven, Stertz Silke, Beibel Martin, Bergling Sebastian, Knehr Judith, Carbone Walter, de Vallière Cheryl, Tchinda Joelle, Bouwmeester Tewis, Seuwen Klaus, Rogler Gerhard, Roma Guglielmo
Abstract excerpt
BACKGROUND: Genome-wide CRISPR-Cas9 dropout screens can identify genes whose knockout affects cell viability. Recent CRISPR screens detected thousands of essential genes required for cellular survival and key cellular processes; however discovering novel lineage-specific genetic dependencies from the many hits still remains a challenge. RESULTS: To assess whether CRISPR-Cas9 dropout screens can help identify...
Topics
- CRISPR-Cas Systems
- Calcium-Calmodulin-Dependent Protein Kinases
- Cell Line, Tumor
- Cell Survival
- Cell Transformation, Neoplastic
- Drug Screening Assays, Antitumor
- Gene Knockout Techniques
- Genome-Wide Association Study
- Humans