Article
Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Sayed Shady, Sürün Duran, Mircetic Jovan, Sidorova Olga Alexandra, Buchholz Frank
Abstract excerpt
The CRISPR-Cas9 technology has revolutionized the scope and pace of biomedical research, enabling the targeting of specific genomic sequences for a wide spectrum of applications. Here we describe assays to functionally interrogate mutations identified in cancer cells utilizing both CRISPR-Cas9 nuclease and base editors. We provide guidelines to interrogate known cancer driver mutations or functionally screen for...
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