Article
Age-Related Accumulation of Somatic Mitochondrial DNA Mutations in Adult-Derived Human iPSCs.
Cell stem cell - 5 May 2016
Kang Eunju, Wang Xinjian, Tippner-Hedges Rebecca, Ma Hong, Folmes Clifford D L, Gutierrez Nuria Marti, Lee Yeonmi, Van Dyken Crystal, Ahmed Riffat, Li Ying, Koski Amy, Hayama Tomonari, Luo Shiyu, Harding Cary O, Amato Paula, Jensen Jeffrey, Battaglia David, Lee David, Wu Diana, Terzic Andre, Wolf Don P, Huang Taosheng, Mitalipov Shoukhrat
Abstract excerpt
The genetic integrity of iPSCs is an important consideration for therapeutic application. In this study, we examine the accumulation of somatic mitochondrial genome (mtDNA) mutations in skin fibroblasts, blood, and iPSCs derived from young and elderly subjects (24-72 years). We found that pooled skin and blood mtDNA contained low heteroplasmic point mutations, but a panel of ten individual iPSC lines from each...
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