Article
Random Mutagenesis, Clonal Events, and Embryonic or Somatic Origin Determine the mtDNA Variant Type and Load in Human Pluripotent Stem Cells.
Stem cell reports - 10 Jul 2018
Zambelli Filippo, Mertens Joke, Dziedzicka Dominika, Sterckx Johan, Markouli Christina, Keller Alexander, Tropel Philippe, Jung Laura, Viville Stephane, Van de Velde Hilde, Geens Mieke, Seneca Sara, Sermon Karen, Spits Claudia
Abstract excerpt
In this study, we deep-sequenced the mtDNA of human embryonic and induced pluripotent stem cells (hESCs and hiPSCs) and their source cells and found that the majority of variants pre-existed in the cells used to establish the lines. Early-passage hESCs carried few and low-load heteroplasmic variants, similar to those identified in oocytes and inner cell masses. The number and heteroplasmic loads of these variants...
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