Article
A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.
Nucleic acids research - 10 Jan 2020
Kesselring Lisa, Miskey Csaba, Zuliani Cecilia, Querques Irma, Kapitonov Vladimir, Laukó Andrea, Fehér Anita, Palazzo Antonio, Diem Tanja, Lustig Janna, Sebe Attila, Wang Yongming, Dinnyés András, Izsvák Zsuzsanna, Barabas Orsolya, Ivics Zoltán
Abstract excerpt
The Sleeping Beauty (SB) transposon is an advanced tool for genetic engineering and a useful model to investigate cut-and-paste DNA transposition in vertebrate cells. Here, we identify novel SB transposase mutants that display efficient and canonical excision but practically unmeasurable genomic re-integration. Based on phylogenetic analyses, we establish compensating amino acid replacements that fully rescue the...
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