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One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

4 Sept 2018

Abstract excerpt

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing prerearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR-Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes...

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PMID: 30181412
DOI: 10.1084/jem.20172064

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