Article
Generation of B cell-deficient pigs by highly efficient CRISPR/Cas9-mediated gene targeting.
Journal of genetics and genomics = Yi chuan xue bao - 20 Aug 2015
Chen Fengjiao, Wang Ying, Yuan Yilin, Zhang Wei, Ren Zijian, Jin Yong, Liu Xiaorui, Xiong Qiang, Chen Qin, Zhang Manling, Li Xiaokang, Zhao Lihua, Li Ze, Wu Zhaoqiang, Zhang Yanfei, Hu Feifei, Huang Juan, Li Rongfeng, Dai Yifan
Abstract excerpt
Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system to target the JH region of the pig IgM heavy chain gene which is crucial for B cell development and differentiation. Transfection of IgM-targeting Cas9 plasmid in primary...
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