Article

One-step generation of monoclonal B cell receptor mice capable of class switch recombination and somatic hypermutation

2017-11-13

Abstract excerpt

We developed a method for rapid generation of B cell receptor (BCR) monoclonal mice expressing pre-rearranged Igh and Igk chains monoallelically from the Igh locus by CRISPR/Cas9 injection into fertilized oocytes. B cells from these mice undergo somatic hypermutation (SHM), class switch recombination (CSR), and affinity-based selection in germinal centers. This method combines the practicality of BCR transgenes...

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Literature Corpus work: bd4927b8-2617-5e82-a864-aabbcf11225f
DOI: 10.1101/218438

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