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Article

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation.

Journal of visualized experiments : JoVE - 2 Feb 2016

Blair John D, Bateup Helen S, Hockemeyer Dirk F

Abstract excerpt

Genome-editing of human pluripotent stem cells (hPSCs) provides a genetically controlled and clinically relevant platform from which to understand human development and investigate the pathophysiology of disease. By employing site-specific nucleases (SSNs) for genome editing, the rapid derivation of new hPSC lines harboring specific genetic alterations in an otherwise isogenic setting becomes possible. Zinc...

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Identifiers

PMID: 26863600
DOI: 10.3791/53583
PMCID: PMC4781717

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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