Article
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation.
Journal of visualized experiments : JoVE - 2 Feb 2016
Blair John D, Bateup Helen S, Hockemeyer Dirk F
Abstract excerpt
Genome-editing of human pluripotent stem cells (hPSCs) provides a genetically controlled and clinically relevant platform from which to understand human development and investigate the pathophysiology of disease. By employing site-specific nucleases (SSNs) for genome editing, the rapid derivation of new hPSC lines harboring specific genetic alterations in an otherwise isogenic setting becomes possible. Zinc...
Topics
- Cell Line
- DNA Breaks, Double-Stranded
- DNA End-Joining Repair
- DNA Repair
- Electroporation
- Endonucleases
- Genome, Human
- Genotype
- Humans
- Pluripotent Stem Cells
- Transgenes