Article
Isolation of single-base genome-edited human iPS cells without antibiotic selection.
Nature methods - 1 Mar 2014
Miyaoka Yuichiro, Chan Amanda H, Judge Luke M, Yoo Jennie, Huang Miller, Nguyen Trieu D, Lizarraga Paweena P, So Po-Lin, Conklin Bruce R
Abstract excerpt
Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of...
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