Article
[Construction of expression vector pLCK-CD69-IRES-EGFP and generation of CD69 transgenic mice].
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences - 1 Sept 2015
Wang Jing, Hu Yan, Tan Bi-qin, Wang Jia-jia, Zhao Meng-ting, Weng Qin-jie, Zhu Di-feng, Wang Hui-ying
Abstract excerpt
OBJECTIVE: To construct the expression vector pLCK-CD69-IRES-EGFP that contains mouse cell surface activation protein CD69 and enhanced green fluorescent protein(EGFP),and to generate CD69 transgenic mice based on this vector. METHODS: First, RNA was extracted from mouse lung tissue and cDNA was synthesized via reverse transcription. PCR primer was designed through the PubMed searching, then mouse CD69 DNA...
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