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[Construction and identification of eukaryotic expression vectors of IKKα carrying Ser176/180 phosphorylation site mutants].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Sept 2015

He Yan, Zheng Qianqian, Zhu Yaqin

Abstract excerpt

OBJECTIVE: To construct eukaryotic expression vectors of the inhibitor of nuclear factor Kappa-B kinaseα (IKKα) containing Ser176/180 phosphorylation site mutants. METHODS: Using PCR, we amplified two IKKα gene fragments, one containing Ser176/180 site mutants and non-sense mutation KpnI and the other with non-sense mutation KpnI. They were subjected to double enzyme digestion, HindIII/KpnI and KpnI/EcoRI,...

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PMID: 26359092

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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