Article
[Construction and function identification of luciferase reporter gene vectors containing SNPs in NFKBIA gene 3'UTR].
Yao xue xue bao = Acta pharmaceutica Sinica - 1 Jan 2016
Yang Shuo, Li Jia-li, Bi Hui-chang, Zhou Shou-ning, Liu Xiao-man, Zeng Hang, Hu Bing-fang, Huang Min
Abstract excerpt
This study aims to investigate the function of two SNPs (rs8904C > T and rs696G >A) in 3' untranslated region (3'UTR) of NFKBIA gene by constructing luciferase reporter gene. A patient's genomic DNA with rs8904 CC and rs696 GA genotype was used as the PCR template. Full-length 3'UTR of NFKBIA gene was amplified by different primers. After sequencing validation, these fragments were inserted to the luciferase...
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