Article
Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.
Genetics - 1 Feb 2016
Bell Ryan T, Fu Becky X H, Fire Andrew Z
Abstract excerpt
The proliferation of CRISPR/Cas9-based methods in Caenorhabditis elegans has enabled efficient genome editing and precise genomic tethering of Cas9 fusion proteins. Experimental designs using CRISPR/Cas9 are currently limited by the need for a protospacer adjacent motif (PAM) in the target with the sequence NGG. Here we report the characterization of two modified Cas9 proteins in C. elegans that recognize NGA and...
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