Article
Sequence-Intrinsic Mechanisms that Target AID Mutational Outcomes on Antibody Genes.
Cell - 19 Nov 2015
Yeap Leng-Siew, Hwang Joyce K, Du Zhou, Meyers Robin M, Meng Fei-Long, Jakubauskaitė Agnė, Liu Mengyuan, Mani Vinidhra, Neuberg Donna, Kepler Thomas B, Wang Jing H, Alt Frederick W
Abstract excerpt
In activated B lymphocytes, AID initiates antibody variable (V) exon somatic hypermutation (SHM) for affinity maturation in germinal centers (GCs) and IgH switch (S) region DNA breaks (DSBs) for class-switch recombination (CSR). To resolve long-standing questions, we have developed an in vivo assay to study AID targeting of passenger sequences replacing a V exon. First, we find AID targets SHM hotspots within V...
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