Article
The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos.
PloS one - 1 Jan 2015
Miyamoto Kei, Suzuki Ken-Ichi T, Suzuki Miyuki, Sakane Yuto, Sakuma Tetsushi, Herberg Sarah, Simeone Angela, Simpson David, Jullien Jerome, Yamamoto Takashi, Gurdon J B
Abstract excerpt
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock-out in founder embryos (F0) needs to be explored in order to judge phenotypes in the F0 generation. Here, we injected modified highly active transcription...
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