Article

Early zygotic engineering promotes targeted large transgene integration and direct production of fully transgenic animals

2024-10-01

Abstract excerpt

Genetic engineering has become increasingly feasible with the advent of site-specific nucleases. This technology is highly effective for the generation of small mutations, however targeted insertion of large sequences to generate transgenic animals remains challenging, time consuming, and laborious. Following several failed attempts using the same reagents, we identified a protocol that achieved very high targeted...

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Literature Corpus work: 12ccc6d1-9a1b-5362-8128-320d6a6b05ac
DOI: 10.1101/2024.09.29.615605

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