Article
TALEN-mediated homologous recombination in Daphnia magna.
Scientific reports - 17 Dec 2015
Nakanishi Takashi, Kato Yasuhiko, Matsuura Tomoaki, Watanabe Hajime
Abstract excerpt
Transcription Activator-Like Effector Nucleases (TALENs) offer versatile tools to engineer endogenous genomic loci in various organisms. We established a homologous recombination (HR)-based knock-in using TALEN in the crustacean Daphnia magna, a model for ecological and toxicological genomics. We constructed TALENs and designed the 67 bp donor insert targeting a point deletion in the eyeless mutant that shows eye...
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