Article
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
Genesis (New York, N.Y. : 2000) - 1 Dec 2013
Nakayama Takuya, Fish Margaret B, Fisher Marilyn, Oomen-Hajagos Jamina, Thomsen Gerald H, Grainger Robert M
Abstract excerpt
We have assessed the efficacy of the recently developed CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated) system for genome modification in the amphibian Xenopus tropicalis. As a model experiment, targeted mutations of the tyrosinase gene were verified, showing the expected albinism phenotype in injected embryos. We further tested this technology by interrupting the six3...
Topics
- Animals
- Brain
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Embryo, Nonmammalian
- Eye
- Eye Proteins
- Genetic Loci
- Genome
- Germ Cells
- Homeodomain Proteins