Article
Generation of a conditional lima1a allele in zebrafish using the FLEx switch technology.
Genesis (New York, N.Y. : 2000) - 1 Jan 2016
Jungke Peggy, Hans Stefan, Gupta Mansi, Machate Anja, Zöller Daniela, Brand Michael
Abstract excerpt
Gene trapping has emerged as a valuable tool to create conditional alleles in various model organisms. Here we report the FLEx-based gene trap vector SAGFLEx that allows the generation of conditional mutations in zebrafish by gene-trap mutagenesis. The SAGFLEx gene-trap cassette comprises the rabbit β-globin splice acceptor and the coding sequence of GFP, flanked by pairs of inversely oriented heterotypic target...
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