Article
Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration.
eLife - 17 Jun 2022
Liu Fang, Kambakam Sekhar, Almeida Maira P, Ming Zhitao, Welker Jordan M, Wierson Wesley A, Schultz-Rogers Laura E, Ekker Stephen C, Clark Karl J, Essner Jeffrey J, McGrail Maura
Abstract excerpt
The ability to regulate gene activity spatially and temporally is essential to investigate cell-type-specific gene function during development and in postembryonic processes and disease models. The Cre/lox system has been widely used for performing cell and tissue-specific conditional analysis of gene function in zebrafish. However, simple and efficient methods for isolation of stable, Cre/lox regulated zebrafish...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.