Article
A versatile gene trap to visualize and interrogate the function of the vertebrate proteome.
Genes & development - 1 Nov 2011
Trinh Le A, Hochgreb Tatiana, Graham Matthew, Wu David, Ruf-Zamojski Frederique, Jayasena Chathurani S, Saxena Ankur, Hawk Rasheeda, Gonzalez-Serricchio Aidyl, Dixson Alana, Chow Elly, Gonzales Constanza, Leung Ho-Yin, Solomon Ilana, Bronner-Fraser Marianne, Megason Sean G, Fraser Scott E
Abstract excerpt
We report a multifunctional gene-trapping approach, which generates full-length Citrine fusions with endogenous proteins and conditional mutants from a single integration event of the FlipTrap vector. We identified 170 FlipTrap zebrafish lines with diverse tissue-specific expression patterns and distinct subcellular localizations of fusion proteins generated by the integration of an internal citrine exon....
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