Article
Conditional gene-trap mutagenesis in zebrafish.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Maddison Lisette A, Li Mingyu, Chen Wenbiao
Abstract excerpt
Zebrafish has become a widely used model for analysis of gene function. Several methods have been used to create mutations in this organism and thousands of mutant lines are available. However, all the conventional zebrafish mutations affect the gene in all cells at all time, making it difficult to determine tissue-specific functions. We have adopted a FlEx Trap approach to generate conditional mutations in...
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