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Article

Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering.

Nucleic acids research - 1 Dec 2012

Lajoie M J, Gregg C J, Mosberg J A, Washington G C, Church G M

Abstract excerpt

Disrupting the interaction between primase and helicase in Escherichia coli increases Okazaki fragment (OF) length due to less frequent primer synthesis. We exploited this feature to increase the amount of ssDNA at the lagging strand of the replication fork that is available for λ Red-mediated Multiplex Automatable Genome Engineering (MAGE). Supporting this concept, we demonstrate that MAGE enhancements correlate...

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Identifiers

PMID: 22904085
DOI: 10.1093/nar/gks751
PMCID: PMC3526312

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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