Article
Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach.
Molecular microbiology - 1 Jul 2004
Yang Hanjing, Wolff Erika, Kim Mandy, Diep Amy, Miller Jeffrey H
Abstract excerpt
We searched for genes that create mutator phenotypes when put on to a multicopy plasmid in Escherichia coli. In many cases, this will result in overexpression of the gene in question. We constructed a random shotgun library with E. coli genomic fragments between 3 and 5 kbp in length on a multicopy plasmid vector that was transformed into E. coli to screen for frameshift mutators. We identified a total of 115...
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