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Coselection of BAC for Escherichia coli chromosomal DNA multiplex automated genome engineering.

Biotechnology letters - 26 Dec 2024

Wang Junyu, Wang Hong, Wang Jiamei, Shang Guangdong

Abstract excerpt

Recombineering (recombination-mediated genetic engineering) is a powerful strategy for bacterial genomic DNA and plasmid DNA modifications. CoS-MAGE improved over MAGE (multiplex automated genome engineering) by co-electroporation of an antibiotic resistance repair oligo along with the oligos for modification of the Escherichia coli chromosome. After several cycles of recombineering, the sub-population of mutants...

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PMID: 39725731
DOI: 10.1007/s10529-024-03554-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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