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Article

Generation of site-specific mutant mice using the CRISPR/Cas9 system.

Yi chuan = Hereditas - 1 Oct 2015

Bai Min, Li Qi, Shao Yan-jiao, Huang Yuan-hua, Li Da-li, Ma Yan-lin

Abstract excerpt

The CRISPR/Cas9 system is a recently developed important technology for genome editing in cellular and animal models. Here we established a CRISPR/Cas9-based system of generating site-specific mutant mice using DNA double-strand breaks (DSBs) induced homologous recombination (HR)-dependent or independent repair mechanism. Through co-microinjection of Cas9 mRNA and single-guide RNA (sgRNA) targeting genomic DNA...

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Source

Identifiers

PMID: 26496755
DOI: 10.16288/j.yczz.15-127

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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