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Article

One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.

Cell - 12 Sept 2013

Yang Hui, Wang Haoyi, Shivalila Chikdu S, Cheng Albert W, Shi Linyu, Jaenisch Rudolf

Abstract excerpt

The type II bacterial CRISPR/Cas system is a novel genome-engineering technology with the ease of multiplexed gene targeting. Here, we created reporter and conditional mutant mice by coinjection of zygotes with Cas9 mRNA and different guide RNAs (sgRNAs) as well as DNA vectors of different sizes. Using this one-step procedure we generated mice carrying a tag or a fluorescent reporter construct in the Nanog, the...

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Identifiers

PMID: 23992847
DOI: 10.1016/j.cell.2013.08.022
PMCID: PMC3961003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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