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Article

Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.

Genome research - 1 Jan 2014

Sung Young Hoon, Kim Jong Min, Kim Hyun-Taek, Lee Jaehoon, Jeon Jisun, Jin Young, Choi Jung-Hwa, Ban Young Ho, Ha Sang-Jun, Kim Cheol-Hee, Lee Han-Woong, Kim Jin-Soo

Abstract excerpt

RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe the establishment of gene-knockout mice and zebrafish by the injection of RGENs as Cas9 protein:guide RNA complexes or Cas9 mRNA plus guide RNA into one-cell-stage embryos of both species. RGENs efficiently generated germline transmittable mutations...

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Identifiers

PMID: 24253447
DOI: 10.1101/gr.163394.113
PMCID: PMC3875853

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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