Article
Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats.
Human genetics - 1 Feb 2021
Yoshimi Kazuto, Oka Yuichiro, Miyasaka Yoshiki, Kotani Yuko, Yasumura Misato, Uno Yoshihiro, Hattori Kosuke, Tanigawa Arisa, Sato Makoto, Oya Manami, Nakamura Kazuhiro, Matsushita Natsuki, Kobayashi Kazuto, Mashimo Tomoji
Abstract excerpt
CRISPR-Cas9 are widely used for gene targeting in mice and rats. The non-homologous end-joining (NHEJ) repair pathway, which is dominant in zygotes, efficiently induces insertion or deletion (indel) mutations as gene knockouts at targeted sites, whereas gene knock-ins (KIs) via homology-directed repair (HDR) are difficult to generate. In this study, we used a double-stranded DNA (dsDNA) donor template with Cas9...
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