Article
Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes.
Development, growth & differentiation - 1 Jan 2014
Mashiko Daisuke, Young Samantha A M, Muto Masanaga, Kato Hirotaka, Nozawa Kaori, Ogawa Masaki, Noda Taichi, Kim Yeon-Joo, Satouh Yuhkoh, Fujihara Yoshitaka, Ikawa Masahito
Abstract excerpt
The recombinant clustered regulatory interspaced short palindromic repeats (CRISPR)/Cas system has opened a new era for mammalian genome editing. Here, we constructed pX330 plasmids expressing humanized Cas9 (hCas9) and single guide RNAs (sgRNAs) against mouse genes and validated them both in vitro and in vivo. When we randomly chose 291 target sequences within protein coding regions of 73 genes, an average...
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