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Article

A high-throughput platform for real-time analysis of membrane fission reactions reveals dynamin function.

Nature cell biology - 1 Dec 2015

Dar Srishti, Kamerkar Sukrut C, Pucadyil Thomas J

Abstract excerpt

Dynamin, the paradigmatic membrane fission catalyst, assembles as helical scaffolds that hydrolyse GTP to sever the tubular necks of clathrin-coated pits. Using a facile assay system of supported membrane tubes (SMrT) engineered to mimic the dimensions of necks of clathrin-coated pits, we monitor the dynamics of a dynamin-catalysed tube-severing reaction in real time using fluorescence microscopy. We find that...

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Identifiers

PMID: 26479317
DOI: 10.1038/ncb3254

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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