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Article

Single Molecule Cluster Analysis dissects splicing pathway conformational dynamics.

Nature methods - 1 Nov 2015

Blanco Mario R, Martin Joshua S, Kahlscheuer Matthew L, Krishnan Ramya, Abelson John, Laederach Alain, Walter Nils G

Abstract excerpt

We report Single Molecule Cluster Analysis (SiMCAn), which utilizes hierarchical clustering of hidden Markov modeling-fitted single-molecule fluorescence resonance energy transfer (smFRET) trajectories to dissect the complex conformational dynamics of biomolecular machines. We used this method to study the conformational dynamics of a precursor mRNA during the splicing cycle as carried out by the spliceosome. By...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 26414013
DOI: 10.1038/nmeth.3602
PMCID: PMC4890712

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